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primary antibody rabbit anti c1q  (R&D Systems)


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    R&D Systems primary antibody rabbit anti c1q
    a, Flow cytometry of CD20-gated B cells from a chronically infected HIV-viremic individual with high-intensity IgG3 on IgM+ B cells, showing binding of <t>C1q</t> and IgG3 on naive B cells and TLM B cells (blue, CRP overlay). Numbers in blue indicate percent CRP among cells in that quadrant. Data are representative of 11 experiments. b, Calcium uptake by C1q+CRP+and C1q−CRP− populations (horizontal axis) of IgG3+IgD+ TLM B cells and naive B cells from chronically infected HIV-viremic individuals (n = 9) with moderate- to high-intensity IgG3 on IgM+ B cells (presented as in Fig. 4b). c, Mean fluorescence intensity of CD32b (evaluated by flow cytometry) on B cells isolated from HIV-viremic individuals (n = 14) and identified as naive, RM, AM or TLM B cells (horizontal axis) by their expression of CD21 and CD27. d, Flow cytometry (left half) showing the expression of IgG3 and IgM by CD20-gated B cells obtained from a chronically infected HIV-viremic individual with high-intensity IgG3 on IgM+ B cells and maintained for 20 min at 4 °C (top) or treated at 37 °C with control goat IgG (bottom left) or goat anti-CD32b (bottom right); middle and right, frequency of IgG3+IgM+ B cells (middle right) and calcium uptake (far right) among B cells obtained from chronically infected HIV-viremic individuals (n = 8 subjects (middle right) or n = 7 subjects (far right)) with moderate- to high-intensity IgG3 on IgM+ B cells and treated with antibody as at bottom left (horizontal axis); results for calcium uptake presented as in b. Each symbol (b–d) represents an individual subject (identified by color in b,c); small horizontal lines (b,c) indicate the median; diagonal lines (d) connect values for the same subject. *P < 0.05, **P < 0.01 and ****P ≤ 0.0001 (two-tailed Wilcoxon matched-pairs signed rank test, after significance was obtained by Friedman ANOVA of the full set (in b,c)).
    Primary Antibody Rabbit Anti C1q, supplied by R&D Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/primary+antibody+rabbit+anti+c1q/C1q+Antibody/pmc06289069-507-12-21
    Average 90 stars, based on 1 article reviews
    primary antibody rabbit anti c1q - by Bioz Stars, 2026-09
    90/100 stars

    Images

    1) Product Images from "IgG3 regulates tissue-like memory B cells in HIV-infected individuals"

    Article Title: IgG3 regulates tissue-like memory B cells in HIV-infected individuals

    Journal: Nature immunology

    doi: 10.1038/s41590-018-0180-5

    a, Flow cytometry of CD20-gated B cells from a chronically infected HIV-viremic individual with high-intensity IgG3 on IgM+ B cells, showing binding of C1q and IgG3 on naive B cells and TLM B cells (blue, CRP overlay). Numbers in blue indicate percent CRP among cells in that quadrant. Data are representative of 11 experiments. b, Calcium uptake by C1q+CRP+and C1q−CRP− populations (horizontal axis) of IgG3+IgD+ TLM B cells and naive B cells from chronically infected HIV-viremic individuals (n = 9) with moderate- to high-intensity IgG3 on IgM+ B cells (presented as in Fig. 4b). c, Mean fluorescence intensity of CD32b (evaluated by flow cytometry) on B cells isolated from HIV-viremic individuals (n = 14) and identified as naive, RM, AM or TLM B cells (horizontal axis) by their expression of CD21 and CD27. d, Flow cytometry (left half) showing the expression of IgG3 and IgM by CD20-gated B cells obtained from a chronically infected HIV-viremic individual with high-intensity IgG3 on IgM+ B cells and maintained for 20 min at 4 °C (top) or treated at 37 °C with control goat IgG (bottom left) or goat anti-CD32b (bottom right); middle and right, frequency of IgG3+IgM+ B cells (middle right) and calcium uptake (far right) among B cells obtained from chronically infected HIV-viremic individuals (n = 8 subjects (middle right) or n = 7 subjects (far right)) with moderate- to high-intensity IgG3 on IgM+ B cells and treated with antibody as at bottom left (horizontal axis); results for calcium uptake presented as in b. Each symbol (b–d) represents an individual subject (identified by color in b,c); small horizontal lines (b,c) indicate the median; diagonal lines (d) connect values for the same subject. *P < 0.05, **P < 0.01 and ****P ≤ 0.0001 (two-tailed Wilcoxon matched-pairs signed rank test, after significance was obtained by Friedman ANOVA of the full set (in b,c)).
    Figure Legend Snippet: a, Flow cytometry of CD20-gated B cells from a chronically infected HIV-viremic individual with high-intensity IgG3 on IgM+ B cells, showing binding of C1q and IgG3 on naive B cells and TLM B cells (blue, CRP overlay). Numbers in blue indicate percent CRP among cells in that quadrant. Data are representative of 11 experiments. b, Calcium uptake by C1q+CRP+and C1q−CRP− populations (horizontal axis) of IgG3+IgD+ TLM B cells and naive B cells from chronically infected HIV-viremic individuals (n = 9) with moderate- to high-intensity IgG3 on IgM+ B cells (presented as in Fig. 4b). c, Mean fluorescence intensity of CD32b (evaluated by flow cytometry) on B cells isolated from HIV-viremic individuals (n = 14) and identified as naive, RM, AM or TLM B cells (horizontal axis) by their expression of CD21 and CD27. d, Flow cytometry (left half) showing the expression of IgG3 and IgM by CD20-gated B cells obtained from a chronically infected HIV-viremic individual with high-intensity IgG3 on IgM+ B cells and maintained for 20 min at 4 °C (top) or treated at 37 °C with control goat IgG (bottom left) or goat anti-CD32b (bottom right); middle and right, frequency of IgG3+IgM+ B cells (middle right) and calcium uptake (far right) among B cells obtained from chronically infected HIV-viremic individuals (n = 8 subjects (middle right) or n = 7 subjects (far right)) with moderate- to high-intensity IgG3 on IgM+ B cells and treated with antibody as at bottom left (horizontal axis); results for calcium uptake presented as in b. Each symbol (b–d) represents an individual subject (identified by color in b,c); small horizontal lines (b,c) indicate the median; diagonal lines (d) connect values for the same subject. *P < 0.05, **P < 0.01 and ****P ≤ 0.0001 (two-tailed Wilcoxon matched-pairs signed rank test, after significance was obtained by Friedman ANOVA of the full set (in b,c)).

    Techniques Used: Flow Cytometry, Infection, Binding Assay, Fluorescence, Isolation, Expressing, Two Tailed Test

    Related Articles

    Incubation:

    Article Title: IgG3 regulates tissue-like memory B cells in HIV-infected individuals
    Article Snippet: .. Membranes were sequentially incubated with goat anti-IgM-HRP (Southern Biotech; Cat# 2020–05) or primary antibody rabbit anti-C1q (Novus; Cat# H00000712-D01P), mouse anti-CRP (R&D Systems; clone 232026), rabbit anti-CD32 (GeneTex; Cat# GTX133371) or rabbit anti- IgG3 (Abcam; clone EPR4419), followed by the secondary antibody, goat anti-rabbit-HRP (Bio-Rad; Cat# 1662408EDU) or goat anti-mouse-HRP (Bio-Rad; Cat# 1000450), diluted in 4% milk powder in 0.1% Tween in PBS. .. B cells (5 × 10 5 ) of HIV-negative individuals were incubated for 20 min at 37 °C with 5 μ g of heat-aggregated IgG3 (Sigma; Cat# I5654) or (Bio-Rad; clone {"type":"entrez-protein","attrs":{"text":"ABD18705","term_id":"87082307","term_text":"ABD18705"}} ABD18705 ), PEG-IgG3, fractions SEC-1 and SEC-2 or agarose-bead-enriched IgG3, isolated from the serum of HIV-viremic individuals.



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    primary antibody rabbit anti c1q  (R&D Systems)
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    R&D Systems primary antibody rabbit anti c1q
    a, Flow cytometry of CD20-gated B cells from a chronically infected HIV-viremic individual with high-intensity IgG3 on IgM+ B cells, showing binding of <t>C1q</t> and IgG3 on naive B cells and TLM B cells (blue, CRP overlay). Numbers in blue indicate percent CRP among cells in that quadrant. Data are representative of 11 experiments. b, Calcium uptake by C1q+CRP+and C1q−CRP− populations (horizontal axis) of IgG3+IgD+ TLM B cells and naive B cells from chronically infected HIV-viremic individuals (n = 9) with moderate- to high-intensity IgG3 on IgM+ B cells (presented as in Fig. 4b). c, Mean fluorescence intensity of CD32b (evaluated by flow cytometry) on B cells isolated from HIV-viremic individuals (n = 14) and identified as naive, RM, AM or TLM B cells (horizontal axis) by their expression of CD21 and CD27. d, Flow cytometry (left half) showing the expression of IgG3 and IgM by CD20-gated B cells obtained from a chronically infected HIV-viremic individual with high-intensity IgG3 on IgM+ B cells and maintained for 20 min at 4 °C (top) or treated at 37 °C with control goat IgG (bottom left) or goat anti-CD32b (bottom right); middle and right, frequency of IgG3+IgM+ B cells (middle right) and calcium uptake (far right) among B cells obtained from chronically infected HIV-viremic individuals (n = 8 subjects (middle right) or n = 7 subjects (far right)) with moderate- to high-intensity IgG3 on IgM+ B cells and treated with antibody as at bottom left (horizontal axis); results for calcium uptake presented as in b. Each symbol (b–d) represents an individual subject (identified by color in b,c); small horizontal lines (b,c) indicate the median; diagonal lines (d) connect values for the same subject. *P < 0.05, **P < 0.01 and ****P ≤ 0.0001 (two-tailed Wilcoxon matched-pairs signed rank test, after significance was obtained by Friedman ANOVA of the full set (in b,c)).
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    Image Search Results


    a, Flow cytometry of CD20-gated B cells from a chronically infected HIV-viremic individual with high-intensity IgG3 on IgM+ B cells, showing binding of C1q and IgG3 on naive B cells and TLM B cells (blue, CRP overlay). Numbers in blue indicate percent CRP among cells in that quadrant. Data are representative of 11 experiments. b, Calcium uptake by C1q+CRP+and C1q−CRP− populations (horizontal axis) of IgG3+IgD+ TLM B cells and naive B cells from chronically infected HIV-viremic individuals (n = 9) with moderate- to high-intensity IgG3 on IgM+ B cells (presented as in Fig. 4b). c, Mean fluorescence intensity of CD32b (evaluated by flow cytometry) on B cells isolated from HIV-viremic individuals (n = 14) and identified as naive, RM, AM or TLM B cells (horizontal axis) by their expression of CD21 and CD27. d, Flow cytometry (left half) showing the expression of IgG3 and IgM by CD20-gated B cells obtained from a chronically infected HIV-viremic individual with high-intensity IgG3 on IgM+ B cells and maintained for 20 min at 4 °C (top) or treated at 37 °C with control goat IgG (bottom left) or goat anti-CD32b (bottom right); middle and right, frequency of IgG3+IgM+ B cells (middle right) and calcium uptake (far right) among B cells obtained from chronically infected HIV-viremic individuals (n = 8 subjects (middle right) or n = 7 subjects (far right)) with moderate- to high-intensity IgG3 on IgM+ B cells and treated with antibody as at bottom left (horizontal axis); results for calcium uptake presented as in b. Each symbol (b–d) represents an individual subject (identified by color in b,c); small horizontal lines (b,c) indicate the median; diagonal lines (d) connect values for the same subject. *P < 0.05, **P < 0.01 and ****P ≤ 0.0001 (two-tailed Wilcoxon matched-pairs signed rank test, after significance was obtained by Friedman ANOVA of the full set (in b,c)).

    Journal: Nature immunology

    Article Title: IgG3 regulates tissue-like memory B cells in HIV-infected individuals

    doi: 10.1038/s41590-018-0180-5

    Figure Lengend Snippet: a, Flow cytometry of CD20-gated B cells from a chronically infected HIV-viremic individual with high-intensity IgG3 on IgM+ B cells, showing binding of C1q and IgG3 on naive B cells and TLM B cells (blue, CRP overlay). Numbers in blue indicate percent CRP among cells in that quadrant. Data are representative of 11 experiments. b, Calcium uptake by C1q+CRP+and C1q−CRP− populations (horizontal axis) of IgG3+IgD+ TLM B cells and naive B cells from chronically infected HIV-viremic individuals (n = 9) with moderate- to high-intensity IgG3 on IgM+ B cells (presented as in Fig. 4b). c, Mean fluorescence intensity of CD32b (evaluated by flow cytometry) on B cells isolated from HIV-viremic individuals (n = 14) and identified as naive, RM, AM or TLM B cells (horizontal axis) by their expression of CD21 and CD27. d, Flow cytometry (left half) showing the expression of IgG3 and IgM by CD20-gated B cells obtained from a chronically infected HIV-viremic individual with high-intensity IgG3 on IgM+ B cells and maintained for 20 min at 4 °C (top) or treated at 37 °C with control goat IgG (bottom left) or goat anti-CD32b (bottom right); middle and right, frequency of IgG3+IgM+ B cells (middle right) and calcium uptake (far right) among B cells obtained from chronically infected HIV-viremic individuals (n = 8 subjects (middle right) or n = 7 subjects (far right)) with moderate- to high-intensity IgG3 on IgM+ B cells and treated with antibody as at bottom left (horizontal axis); results for calcium uptake presented as in b. Each symbol (b–d) represents an individual subject (identified by color in b,c); small horizontal lines (b,c) indicate the median; diagonal lines (d) connect values for the same subject. *P < 0.05, **P < 0.01 and ****P ≤ 0.0001 (two-tailed Wilcoxon matched-pairs signed rank test, after significance was obtained by Friedman ANOVA of the full set (in b,c)).

    Article Snippet: Membranes were sequentially incubated with goat anti-IgM-HRP (Southern Biotech; Cat# 2020–05) or primary antibody rabbit anti-C1q (Novus; Cat# H00000712-D01P), mouse anti-CRP (R&D Systems; clone 232026), rabbit anti-CD32 (GeneTex; Cat# GTX133371) or rabbit anti- IgG3 (Abcam; clone EPR4419), followed by the secondary antibody, goat anti-rabbit-HRP (Bio-Rad; Cat# 1662408EDU) or goat anti-mouse-HRP (Bio-Rad; Cat# 1000450), diluted in 4% milk powder in 0.1% Tween in PBS.

    Techniques: Flow Cytometry, Infection, Binding Assay, Fluorescence, Isolation, Expressing, Two Tailed Test